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Nacalai extra page one precast gel
Extra Page One Precast Gel, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extra+page+one+precast+gel/extra+gel+one+page+precast/pmc13128273-254-9-14
Average 86 stars, based on 1 article reviews
extra page one precast gel - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

SDS Page:

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Article Title: Identification of Paramyosin as a Novel Allergen in Edible Cricket Protein.
Article Snippet: .. The proteins were then separated by SDS-PAGE using Extra PAGE One precast gel (7.5–15%, Nacalai Tesque, Inc., Kyoto, Japan). .. Following electrophoresis, the proteins were either stained with Coomassie Brilliant Blue (CBB) R-250 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) or transferred onto a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotting system (ATTO, Tokyo, Japan).

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid.
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Polyacrylamide Gel Electrophoresis:

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Article Title: C-Terminal End of Gli3 Is Critical for Functional Protein Synthesis and Gli3-Dependent Anatomical Development.
Article Snippet: .. An equal amount of cell lysates was then applied to a 7.5% Extra PAGE One Precast gel (Nacalai Tesque) and separated through electrophoresis. ..

Article Title: Ghrelin restores D1 receptor-mediated dopamine neurotransmission and enhances attentive behaviour in Mecp2 KO mice.
Article Snippet: Rett syndrome is an X-linked neurodevelopmental disorder characterised by cognitive, attentional, and communicative impairments along with sensory and motor deficits.. Optimal activation of dopamine D1 receptor signalling in the prefrontal cortex (PFC) is essential for cognition and object-based attention.. Ghrelin has been observed to modulate dopaminergic neurotransmission and improve cognitive function and attention impairments in various animal models.

Article Title: The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism
Article Snippet: The protein lysate was mixed with an equal volume of 3×SDS sample buffer (187.5 mM Tris-HCl [pH 6.8], 30% [v/v] glycerol [Nacalai Tesque], 6% [w/v] SDS, 0.3 M DTT [Nacalai Tesque], 0.02% (w/v) bromophenol blue [Nacalai Tesque]) and boiled at 100°C for 5 min. .. Equal amounts of total protein were loaded onto 5–20% Extra PAGE One Precast Gel (Nacalai Tesque) and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). .. For wet transfer, proteins in the gels were transferred onto 0.45 μm pore Immobilon-FL polyvinylidene difluoride (PVDF) transfer membranes (Merck Millipore) using 10 mM CAPS buffer (3-[cyclohexylamino] propane-1-sulfonic acid [Nacalai Tesque], pH 11.0) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) at 4°C at 50 V for 14 h. For semi-dry transfer, proteins were transferred onto 0.45 μm pore Immobilon-FL PVDF transfer membranes using semi-dry buffer (24 mM Tris-HCl, 0.1% [w/v] SDS, 192 mM glycine [Nacalai Tesque], 20% [v/v] ethanol [Nacalai Tesque]) in Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) at room temperature, at 10 V for 1 h. After the transfer, the membranes were incubated with 1×Tris-NaCl-Tween (TNT) buffer (0.1 M Tris-HCl [pH 7.5], 140 mM NaCl, 0.1% [v/v] Tween 20 [Nacalai Tesque]) containing 3% [w/v] skim milk (MEGMILK SNOW BRAND, Tokyo, Japan) for 30 min. After washing the membranes with 1×TNT buffer four times for 5 min each, the membranes were incubated with relevant primary antibodies at 4°C overnight.

Article Title: The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism
Article Snippet: The protein lysate was mixed with an equal volume of 3× SDS sample buffer (187.5 mM Tris–HCl [pH 6.8], 30% [v/v] glycerol [Nacalai Tesque], 6% [w/v] SDS, 0.3 M DTT [Nacalai Tesque], 0.02% (w/v) bromophenol blue [Nacalai Tesque]) and boiled at 100°C for 5 min. .. Equal amounts of total protein were loaded onto 5%–20% Extra PAGE One Precast Gel (Nacalai Tesque) and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). .. For wet transfer, proteins in the gels were transferred onto 0.45 μm pore Immobilon-FL polyvinylidene difluoride (PVDF) transfer membranes (Merck Millipore) using 10 mM CAPS buffer (3-[cyclohexylamino] propane-1-sulfonic acid [Nacalai Tesque], pH 11.0) in a Mini Trans-Blot Electrophoretic Transfer Cell Tank (Bio-Rad) at 4°C at 50 V for 14 h. For semi-dry transfer, proteins were transferred onto 0.45 μm pore Immobilon-FL PVDF transfer membranes using semi-dry buffer (24 mM Tris–HCl, 0.1% [w/v] SDS, 192 mM glycine [Nacalai Tesque], 20% [v/v] ethanol [Nacalai Tesque]) in Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) at room temperature, at 10 V for 1 h. After the transfer, the membranes were incubated with 1× Tris–NaCl–Tween (TNT) buffer (0.1 M Tris–HCl [pH 7.5], 140 mM NaCl, 0.1% [v/v] Tween 20 [Nacalai Tesque]) containing 3% [w/v] skim milk (MEGMILK SNOW BRAND, Tokyo, Japan) for 30 min. After washing the membranes with 1× TNT buffer four times for 5 min each, the membranes were incubated with relevant primary antibodies at 4°C overnight.

Article Title: Identification of Paramyosin as a Novel Allergen in Edible Cricket Protein.
Article Snippet: .. The proteins were then separated by SDS-PAGE using Extra PAGE One precast gel (7.5–15%, Nacalai Tesque, Inc., Kyoto, Japan). .. Following electrophoresis, the proteins were either stained with Coomassie Brilliant Blue (CBB) R-250 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) or transferred onto a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotting system (ATTO, Tokyo, Japan).

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid.
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Article Title: Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures
Article Snippet: .. The samples were loaded onto an Extra PAGE One Precast Gel (Nacalai Tesque), along with either Precision Plus Protein Dual Color Standard markers (Bio-Rad) or ExcelBand All Blue Broad Range Plus Protein Marker (SMOBIO), and electrophoresis was performed. .. Proteins in the gel were transferred onto an Immobilon-P Transfer Membrane (Merck Millipore) treated with methanol.

Membrane:

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Article Title: SLC25A11 Is Associated with KDM2A-Dependent Reduction in rRNA Transcription Induced by Aminooxyacetic Acid.
Article Snippet: .. The lysates were subjected to SDS-PAGE using an Extra PAGE One Precast Gel (Nacalai Tesque; # 13062-84) or self-made gel and transferred onto a polyvinylidene fluoride membrane (Millipore, Burlington, MA, USA; #IPVH00010). ..

Electrophoresis:

Article Title: C-Terminal End of Gli3 Is Critical for Functional Protein Synthesis and Gli3-Dependent Anatomical Development.
Article Snippet: .. An equal amount of cell lysates was then applied to a 7.5% Extra PAGE One Precast gel (Nacalai Tesque) and separated through electrophoresis. ..

Article Title: Ghrelin restores D1 receptor-mediated dopamine neurotransmission and enhances attentive behaviour in Mecp2 KO mice.
Article Snippet: Rett syndrome is an X-linked neurodevelopmental disorder characterised by cognitive, attentional, and communicative impairments along with sensory and motor deficits.. Optimal activation of dopamine D1 receptor signalling in the prefrontal cortex (PFC) is essential for cognition and object-based attention.. Ghrelin has been observed to modulate dopaminergic neurotransmission and improve cognitive function and attention impairments in various animal models.

Article Title: Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures
Article Snippet: .. The samples were loaded onto an Extra PAGE One Precast Gel (Nacalai Tesque), along with either Precision Plus Protein Dual Color Standard markers (Bio-Rad) or ExcelBand All Blue Broad Range Plus Protein Marker (SMOBIO), and electrophoresis was performed. .. Proteins in the gel were transferred onto an Immobilon-P Transfer Membrane (Merck Millipore) treated with methanol.

Nucleic Acid Electrophoresis:

Article Title: The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism
Article Snippet: The protein lysate was mixed with an equal volume of 3×SDS sample buffer (187.5 mM Tris-HCl [pH 6.8], 30% [v/v] glycerol [Nacalai Tesque], 6% [w/v] SDS, 0.3 M DTT [Nacalai Tesque], 0.02% (w/v) bromophenol blue [Nacalai Tesque]) and boiled at 100°C for 5 min. .. Equal amounts of total protein were loaded onto 5–20% Extra PAGE One Precast Gel (Nacalai Tesque) and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). .. For wet transfer, proteins in the gels were transferred onto 0.45 μm pore Immobilon-FL polyvinylidene difluoride (PVDF) transfer membranes (Merck Millipore) using 10 mM CAPS buffer (3-[cyclohexylamino] propane-1-sulfonic acid [Nacalai Tesque], pH 11.0) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) at 4°C at 50 V for 14 h. For semi-dry transfer, proteins were transferred onto 0.45 μm pore Immobilon-FL PVDF transfer membranes using semi-dry buffer (24 mM Tris-HCl, 0.1% [w/v] SDS, 192 mM glycine [Nacalai Tesque], 20% [v/v] ethanol [Nacalai Tesque]) in Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) at room temperature, at 10 V for 1 h. After the transfer, the membranes were incubated with 1×Tris-NaCl-Tween (TNT) buffer (0.1 M Tris-HCl [pH 7.5], 140 mM NaCl, 0.1% [v/v] Tween 20 [Nacalai Tesque]) containing 3% [w/v] skim milk (MEGMILK SNOW BRAND, Tokyo, Japan) for 30 min. After washing the membranes with 1×TNT buffer four times for 5 min each, the membranes were incubated with relevant primary antibodies at 4°C overnight.

Gel Electrophoresis:

Article Title: The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism
Article Snippet: The protein lysate was mixed with an equal volume of 3× SDS sample buffer (187.5 mM Tris–HCl [pH 6.8], 30% [v/v] glycerol [Nacalai Tesque], 6% [w/v] SDS, 0.3 M DTT [Nacalai Tesque], 0.02% (w/v) bromophenol blue [Nacalai Tesque]) and boiled at 100°C for 5 min. .. Equal amounts of total protein were loaded onto 5%–20% Extra PAGE One Precast Gel (Nacalai Tesque) and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). .. For wet transfer, proteins in the gels were transferred onto 0.45 μm pore Immobilon-FL polyvinylidene difluoride (PVDF) transfer membranes (Merck Millipore) using 10 mM CAPS buffer (3-[cyclohexylamino] propane-1-sulfonic acid [Nacalai Tesque], pH 11.0) in a Mini Trans-Blot Electrophoretic Transfer Cell Tank (Bio-Rad) at 4°C at 50 V for 14 h. For semi-dry transfer, proteins were transferred onto 0.45 μm pore Immobilon-FL PVDF transfer membranes using semi-dry buffer (24 mM Tris–HCl, 0.1% [w/v] SDS, 192 mM glycine [Nacalai Tesque], 20% [v/v] ethanol [Nacalai Tesque]) in Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) at room temperature, at 10 V for 1 h. After the transfer, the membranes were incubated with 1× Tris–NaCl–Tween (TNT) buffer (0.1 M Tris–HCl [pH 7.5], 140 mM NaCl, 0.1% [v/v] Tween 20 [Nacalai Tesque]) containing 3% [w/v] skim milk (MEGMILK SNOW BRAND, Tokyo, Japan) for 30 min. After washing the membranes with 1× TNT buffer four times for 5 min each, the membranes were incubated with relevant primary antibodies at 4°C overnight.

Marker:

Article Title: Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures
Article Snippet: .. The samples were loaded onto an Extra PAGE One Precast Gel (Nacalai Tesque), along with either Precision Plus Protein Dual Color Standard markers (Bio-Rad) or ExcelBand All Blue Broad Range Plus Protein Marker (SMOBIO), and electrophoresis was performed. .. Proteins in the gel were transferred onto an Immobilon-P Transfer Membrane (Merck Millipore) treated with methanol.



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